10927 Tradd Status: Cancelled
| Gene | Tradd |
| Chromosome | 8 |
| Deletion Start | 105,260,669 |
| Deletion End | 105,258,950 |
| Deletion Size | 1,720 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,260,484 | 105,260,506 | GGTGTAGTGACTCTGGGACTTTG |
| TUP | 105,260,449 | 105,260,474 | CTGGGATTGAGGGCCCACTTCTTTGC |
| TUR | 105,260,397 | 105,260,419 | CACCTCACCCAATAGTCTTTCTC |
| TDF | 105,259,556 | 105,259,574 | TGACGTGTGACTGCACTGG |
| TDP | 105,259,529 | 105,259,554 | CAGGGTGGAGCCATACAGGTAGCTTC |
| TDR | 105,259,491 | 105,259,509 | CCTCGGTCGGAGAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCCGTGTATAGGAGGAGCAG |
| SD | TTATCGCTGCAGACAAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
