10930 Isg20 Status: Cancelled
| Gene | Isg20 |
| Chromosome | 7 |
| Deletion Start | 78,914,326 |
| Deletion End | 78,920,159 |
| Deletion Size | 5,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,914,564 | 78,914,587 | CCAGGCTAGAGGTAAGTGAAGAAG |
| TUP | 78,914,589 | 78,914,613 | AGCAGGAGTCGAGGCACATTTGTCC |
| TUR | 78,914,639 | 78,914,658 | GGGAGAGTCACAGACCACAG |
| TDF | 78,919,887 | 78,919,907 | GCTCGCACGCTGTTATTTCTG |
| TDP | 78,919,908 | 78,919,931 | CCCTGTCCAGCTCTAGTCACCTGC |
| TDR | 78,919,944 | 78,919,963 | AGGTCCCTGACTCGGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | AACCACATAGCTGCCCAAAC |
| SD | TTCCTATTACTGCCATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 493 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
