10933 Kap Status: Cancelled
| Gene | Kap |
| Chromosome | 6 |
| Deletion Start | 133,853,620 |
| Deletion End | 133,850,031 |
| Deletion Size | 3,590 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,853,144 | 133,853,163 | TGTGGGTCTCGGATAGGAAC |
| TUP | 133,853,119 | 133,853,142 | CAGGCTGTTTCTCAGGAGAGCTGC |
| TUR | 133,853,093 | 133,853,113 | CCGGTCATTTGCACACAGTTG |
| TDF | 133,850,393 | 133,850,413 | TGAACCTGGGTCCATTGGAAG |
| TDP | 133,850,366 | 133,850,392 | AGCAGCCTCTAGTGCTAAGTGCTGAGC |
| TDR | 133,850,346 | 133,850,365 | CAGGAAGGGTTGGAGCGATG |
PCR Primers
| Primer | Sequence |
| SU | AGATGACCCCTGGCCATTAG |
| SD | ACAATTATTGGATCCTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 449 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 393 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
