10934 Arsi Status: Cancelled
| Gene | Arsi |
| Chromosome | 18 |
| Deletion Start | 60,912,243 |
| Deletion End | 60,917,772 |
| Deletion Size | 5,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,912,639 | 60,912,657 | GCACGGACCTGAGAAACAC |
| TUP | 60,912,661 | 60,912,682 | TTACGAGCCAGCTTTGCCTGCA |
| TUR | 60,912,694 | 60,912,715 | GGGCAAGGGACTTATGGATGAG |
| TDF | 60,917,291 | 60,917,310 | GCAGGCTGCTATCCGAGTTG |
| TDP | 60,917,314 | 60,917,338 | AGTGGAAGCTGCTCACTGGAGACCC |
| TDR | 60,917,354 | 60,917,374 | GCCAGTGTCTGAGGTGGAATC |
PCR Primers
| Primer | Sequence |
| SU | CCGGGGATGGTGGAGAGCAC |
| SD | AGTCCTAAAGTCACCAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
