10935 Zcchc5 Status: Cancelled
| Gene | Zcchc5 |
| Chromosome | X |
| Deletion Start | 106,839,651 |
| Deletion End | 106,837,957 |
| Deletion Size | 1,695 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,838,965 | 106,838,984 | TTCAGAGTCCACAGCTCCAC |
| TUP | 106,838,933 | 106,838,962 | CCCTACAAATGATGAGGAGTCTCAGACGGT |
| TUR | 106,838,907 | 106,838,928 | GTGATGAGGTCTCCTGGTATTC |
| TDF | 106,838,598 | 106,838,616 | GCGGCTCTAGTGAGCTTTG |
| TDP | 106,838,573 | 106,838,596 | TGGCAGCTTCTTCTCAGGTGAGGC |
| TDR | 106,838,540 | 106,838,563 | TGGCTATCTAATAAGGGCTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | ATAGAATGAGTGCTTTCCAC |
| SD | CAAACTGGAGCTCTTCAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 383 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
