10936 Dnd1 Status: Cancelled
| Gene | Dnd1 |
| Chromosome | 18 |
| Deletion Start | 36,766,163 |
| Deletion End | 36,763,931 |
| Deletion Size | 2,252 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,766,138 | 36,766,158 | CAAACGGGAGTGCGAGGTAAG |
| TUP | 36,766,105 | 36,766,131 | AAGACCTAAGTGATTGTTGGGCACGGC |
| TUR | 36,766,081 | 36,766,101 | CACCGTAACCATCCCCTTCTG |
| TDF | 36,764,607 | 36,764,630 | GGAAGTTGTCACACCACCTAGAAG |
| TDP | 36,764,578 | 36,764,606 | TGTAGAGGCATTAAACATAGCCTGTGGCA |
| TDR | 36,764,510 | 36,764,529 | GCAGAGAGACAAGGGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTGCTAGAGGTTCTTGTTTC |
| SD | CAAGCAGACACATGTATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 388 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
