10937 1700086D15Rik Status: Cancelled
| Gene | 1700086D15Rik |
| Chromosome | 11 |
| Deletion Start | 65,153,363 |
| Deletion End | 65,152,476 |
| Deletion Size | 888 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,153,207 | 65,153,228 | GTGGAAGAACAGGACACAAGAC |
| TUP | 65,153,181 | 65,153,204 | TGGTGAGTCACACTCTGGCTTCCA |
| TUR | 65,153,134 | 65,153,155 | GGCAATGAAATGGCACAAATGG |
| TDF | 65,152,890 | 65,152,911 | GCCAGACTTGATACCTCCTGAG |
| TDP | 65,152,865 | 65,152,888 | AGAGCAAACCTGGGCTGAGGCTTC |
| TDR | 65,152,822 | 65,152,841 | GGGACATTGTGGCTGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GACACTCTATAGGATTATTG |
| SD | GGGACCTGGGTGCTCTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
