10944 Rpa3 Status: ES cell colonies screened / QC no positives
| Gene | Rpa3 |
| Chromosome | 6 |
| Deletion Start | 8,259,095 |
| Deletion End | 8,255,845 |
| Deletion Size | 3,251 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,258,706 | 8,258,726 | GGCTCCTCTGTCTGTTGTTCG |
| TUP | 8,258,669 | 8,258,696 | TTTAGCCAGACTTAGCTTCTTGCTTCCC |
| TUR | 8,258,609 | 8,258,629 | AGGGAGACGACTGCAAAGTAG |
| TDF | 8,256,776 | 8,256,800 | GACGAGGAAATCTCTGGGATTGTAG |
| TDP | 8,256,746 | 8,256,771 | AGTTGGAAAAGTCACAGCCAAGGCGA |
| TDR | 8,256,720 | 8,256,743 | CAGGGTATAAGATGCACAGAGCAC |
PCR Primers
| Primer | Sequence |
| SU | CCCCAGTGACTTCCGGAAC |
| SD | CAAAGATTCTGCCAACCCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 359 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
