10947 Usp-ps Status: Cancelled
| Gene | Usp-ps |
| Chromosome | 7 |
| Deletion Start | 104,670,707 |
| Deletion End | 104,672,016 |
| Deletion Size | 1,310 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,671,086 | 104,671,105 | TCTGCTACCCTCTGCACTAC |
| TUP | 104,671,107 | 104,671,135 | CCACTATCCTCACCAATCCTGTCATTGCA |
| TUR | 104,671,152 | 104,671,172 | GCACCACAGCTCTCAAGAATG |
| TDF | 104,671,536 | 104,671,559 | TCATTGTGGCTAACCTTTATCTGC |
| TDP | 104,671,563 | 104,671,586 | TGCCTCCCACTATGAACCCTGTGG |
| TDR | 104,671,603 | 104,671,625 | TGACACAGTCTCGTATCTGCTTG |
PCR Primers
| Primer | Sequence |
| SU | GTTAGAAATGTGAGTGTGGAAG |
| SD | CCTCCACATATTTGTTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 458 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 399 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
