10948 Nxt2 Status: ES cell colonies screened / QC no positives
| Gene | Nxt2 |
| Chromosome | X |
| Deletion Start | 142,228,370 |
| Deletion End | 142,237,848 |
| Deletion Size | 9,496 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,229,202 | 142,229,219 | TTGCCCACACTTCACACC |
| TUP | 142,229,220 | 142,229,240 | TAGGGTCCCTGCCTCTGCACA |
| TUR | 142,229,248 | 142,229,271 | CTTTGCTTGGCATGTATCTGTAAC |
| TDF | 142,237,650 | 142,237,672 | AGCTACCCAGTGCCAAACTACAG |
| TDP | 142,237,673 | 142,237,697 | TGCTCGTTGTGACCAGTGGAGTTGT |
| TDR | 142,237,732 | 142,237,752 | CGCAGTCAGCAGGAAGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGACAGGTAGCTGGGAAC |
| SD | AACAGGCATGTGCTTCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 550 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 466 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
