| Gene | Zcchc12 |
| Chromosome | X |
| Deletion Start | 36,197,415 |
| Deletion End | 36,198,656 |
| Deletion Size | 1,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,197,621 | 36,197,640 | TGGCTGAGCCAAGTAACTGG |
| TUP | 36,197,657 | 36,197,685 | CATATGCCTGAGGAGGAAAAGGTCAGACG |
| TUR | 36,197,727 | 36,197,747 | GATTGGCAGCTTGGAGCAAAC |
| TDF | 36,198,445 | 36,198,465 | AGCCAGGAAGCGCAAACATAC |
| TDP | 36,198,468 | 36,198,492 | TCCATTGTTCCCACTGTGGTGAGGA |
| TDR | 36,198,517 | 36,198,536 | GGCCCTTGTCACTCTCATTG |
PCR Primers
| Primer | Sequence |
| SU | GAGGGTATGGATGACGTGGC |
| SD | CACAAGGTAGAGAGGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 417 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 398 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10951A-B8 | VGB6 | C57BL/6NTac | |
| 10951A-D12 | VGB6 | C57BL/6NTac | |
