10952 Tex13 Status: ES cell colonies screened / QC one positive
| Gene | Tex13 |
| Chromosome | X |
| Deletion Start | 140,813,087 |
| Deletion End | 140,810,034 |
| Deletion Size | 3,054 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,812,736 | 140,812,755 | CACCGACCAGCACGAGATAG |
| TUP | 140,812,713 | 140,812,735 | AACGCAAGGAGGCGGCCTACCAG |
| TUR | 140,812,679 | 140,812,700 | GCACCTCTGCGAGTTTAGTGTG |
| TDF | 140,810,485 | 140,810,504 | GGCCTAGATTGGCCTCAAAC |
| TDP | 140,810,450 | 140,810,478 | TGTAGCTGACTCCAGATCCTTTTGCCTCC |
| TDR | 140,810,419 | 140,810,440 | ACTGCCCTGTCAATTCCAACAC |
PCR Primers
| Primer | Sequence |
| SU | TTCAGAGAGGTGAGGCCTGG |
| SD | TGAGCGAACCAGACTGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 382 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
