10958 Josd2 Status: Cancelled
| Gene | Josd2 |
| Chromosome | 7 |
| Deletion Start | 44,468,827 |
| Deletion End | 44,471,521 |
| Deletion Size | 2,695 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,468,909 | 44,468,931 | TCAACAACGTCCTTCAAGAGCAG |
| TUP | 44,468,933 | 44,468,954 | TCTTTAGCCAGGAGGCTGCCGA |
| TUR | 44,468,967 | 44,468,986 | GGAACAATGGCAGTCACCTC |
| TDF | 44,470,711 | 44,470,735 | GGGAAAGACAGAAGTTAGTCAAGAG |
| TDP | 44,470,739 | 44,470,760 | TGGAGCCATCTTGTTGGCCCAA |
| TDR | 44,470,768 | 44,470,786 | AGGCTGGCTTCCCGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | GAGACGGGAAAGGAGGAGTC |
| SD | CCTGTCTGCTGCCTAGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
