10959 A230051G13Rik Status: ES cell colonies screened / QC no positives
| Gene | A230051G13Rik |
| Chromosome | 11 |
| Deletion Start | 59,163,688 |
| Deletion End | 59,158,171 |
| Deletion Size | 5,518 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,163,140 | 59,163,158 | TCACCTAGAGGCGGATGTC |
| TUP | 59,163,115 | 59,163,138 | CATGGAAGGCATCCAGGTTGCGCT |
| TUR | 59,163,094 | 59,163,113 | CAACGCTCTGTGCAAGTAAC |
| TDF | 59,158,351 | 59,158,371 | TGCGGTCAGAGACAATCAAGG |
| TDP | 59,158,326 | 59,158,349 | TCCTCTCCACATCAAGACCTCCGA |
| TDR | 59,158,302 | 59,158,319 | GCAGTCACAGCCACCAAC |
PCR Primers
| Primer | Sequence |
| SU | AACCGTGAACAAGGAAATGC |
| SD | CTCTGCAGGCACACAGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
