10961 Spata2l Status: Cancelled
| Gene | Spata2l |
| Chromosome | 8 |
| Deletion Start | 123,235,817 |
| Deletion End | 123,233,028 |
| Deletion Size | 2,790 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,235,506 | 123,235,530 | CACCACTATCAAGGTATGGCATCAG |
| TUP | 123,235,477 | 123,235,499 | TCCTTCACTCCCGTCCTTGTCCA |
| TUR | 123,235,421 | 123,235,440 | ACGAGCCCTCAACGCAGAAC |
| TDF | 123,233,386 | 123,233,403 | CCACTGCACTGCCCTATC |
| TDP | 123,233,366 | 123,233,385 | TGCCTGCCGCCCGACTCATT |
| TDR | 123,233,327 | 123,233,347 | ACAGGCGTCTCTGGTTGTCAC |
PCR Primers
| Primer | Sequence |
| SU | ATACGAGGACGCTCAGCAAG |
| SD | CCTCACAGCTAAGTAAGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
