| Gene | Zc3hav1l |
| Chromosome | 6 |
| Deletion Start | 38,299,237 |
| Deletion End | 38,293,022 |
| Deletion Size | 6,216 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,298,618 | 38,298,640 | GCTAGGATGTTCCACAACAGCAG |
| TUP | 38,298,595 | 38,298,617 | CCCGGTTGAATCCGTGTTGCCAC |
| TUR | 38,298,564 | 38,298,585 | CAAGCGGCATTTGGCATAAGTG |
| TDF | 38,293,936 | 38,293,958 | TGAGCTTAGTTCCCAGCTATCAC |
| TDP | 38,293,907 | 38,293,931 | TGGCTCACACCCATCTGTAATGGGA |
| TDR | 38,293,878 | 38,293,897 | TCACTGTCACACCAGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | AAAGGACGCTGGCCTTGATC |
| SD | TCACCCTGGATTACGTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10965A-G4 | VGB6 | C57BL/6NTac | |
| 10965B-A1 | VGB6 | C57BL/6NTac | |
| 10965B-G1 | VGB6 | C57BL/6NTac | |
| 10965B-E12 | VGB6 | C57BL/6NTac | |
