10966 Phospho1 Status: Cancelled
| Gene | Phospho1 |
| Chromosome | 11 |
| Deletion Start | 95,828,734 |
| Deletion End | 95,831,306 |
| Deletion Size | 2,573 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,829,030 | 95,829,050 | GCCAAGCTCCAATACCAACTC |
| TUP | 95,829,053 | 95,829,076 | AAATGAGGTGTGCCAGGCCCAGAA |
| TUR | 95,829,089 | 95,829,109 | GACAGCATCCTAAGCCTCACC |
| TDF | 95,830,383 | 95,830,403 | GCAGCACATTGGTTCCATCTC |
| TDP | 95,830,405 | 95,830,429 | TGCTTAACCAGCCTTGGGTCCTCCT |
| TDR | 95,830,433 | 95,830,450 | CGCTGGCACATCCAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGGAAGCCAGTGGGCAGGC |
| SD | AACCCGTTCCTCTCAGTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 409 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 379 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
