10970   Cypt2     Status: ES cell colonies screened / QC no positives

GeneCypt2
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:2676331
NCBI Gene ID245566
BAC ID260M17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGCCAAGAAAGTCCACTGGTC
TUPAGCAGCTACAGCAGTAGCCGCCA
TURGCTTGAATCTTTTGGCAGCAG
TDFGAGACGAATCAAGCGTGCTAC
TDPTGAACTTCGATTGATGCAGAGCCACC
TDRGCTCGGTTCCAGTTCGATGAG

PCR Primers

PrimerSequence
SUTTCCAGAGGTTTAAGTAAAG
SDTTTGCTACCTCAGTTCTGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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