10978 Ddit4l Status: Cancelled
| Gene | Ddit4l |
| Chromosome | 3 |
| Deletion Start | 137,624,186 |
| Deletion End | 137,626,551 |
| Deletion Size | 2,385 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,624,213 | 137,624,231 | AACCCGGCCAGCATTTCAG |
| TUP | 137,624,234 | 137,624,256 | TTGTTGGACGGTGGCTATCACCC |
| TUR | 137,624,267 | 137,624,287 | GCCCAGAGACTCACCACTTAG |
| TDF | 137,626,086 | 137,626,108 | CCAAACTTGGTTGCTCTAAGGTC |
| TDP | 137,626,110 | 137,626,133 | TGGTCCCTGAGAAACTGACCCAGA |
| TDR | 137,626,140 | 137,626,160 | AGAGCCGCAGGACATCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGAGGCGGGACCTATAAAGC |
| SD | TACAATAACAGCAAGCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 623 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
