10979 Ube2q2 Status: Cancelled
| Gene | Ube2q2 |
| Chromosome | 9 |
| Deletion Start | 55,149,638 |
| Deletion End | 55,180,609 |
| Deletion Size | 30,991 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,150,409 | 55,150,426 | TGCAAGCTGTCCCACCTG |
| TUP | 55,150,431 | 55,150,454 | CTGTGCGGAAGCCTGGACGTTACC |
| TUR | 55,150,493 | 55,150,517 | AGCCTTCCAATCCCACTTGATCTTC |
| TDF | 55,180,263 | 55,180,283 | TGAGGCTGTGGGATCTGCTTT |
| TDP | 55,180,284 | 55,180,303 | CCACAGAACATAAGGTATTA |
| TDR | 55,180,311 | 55,180,332 | CCAGACTCCACTCACAAGGTTT |
PCR Primers
| Primer | Sequence |
| SU | GTTCATCCTCGTGCGGTG |
| SD | GATTCTTATACAGAGACAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 621 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
