10982 Tubb4a Status: Cancelled
| Gene | Tubb4a |
| Chromosome | 17 |
| Deletion Start | 57,087,510 |
| Deletion End | 57,080,593 |
| Deletion Size | 6,937 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,087,007 | 57,087,025 | AGCCCTTCCCGACTAACAC |
| TUP | 57,086,978 | 57,087,006 | TGTGACCCACTCTGACATCTTAGCACTTG |
| TUR | 57,086,958 | 57,086,977 | GGGCTCCTGCCTCTATCCAC |
| TDF | 57,080,699 | 57,080,720 | CGAAGAGGAGGCTGAAGAGGAG |
| TDP | 57,080,671 | 57,080,697 | TGGCTTAAGTCTCCTGCCATCACTCTG |
| TDR | 57,080,621 | 57,080,640 | TGCAGGGGTGTGATGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCATTACTCCAGTCCCTC |
| SD | TCTGGGCATGTCAGAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
