10986 Mesdc1 Status: ES cell colonies screened / QC no positives
| Gene | Mesdc1 |
| Chromosome | 7 |
| Deletion Start | 83,883,218 |
| Deletion End | 83,882,074 |
| Deletion Size | 1,145 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,882,845 | 83,882,862 | GTCGCTGACCGAGTGTTC |
| TUP | 83,882,820 | 83,882,841 | ACATGCGGCCTACCTGGCAGCC |
| TUR | 83,882,722 | 83,882,741 | AGCACAGCACAGCCTTGTTC |
| TDF | 83,882,248 | 83,882,266 | TGTCGGACCACAGGGAGAG |
| TDP | 83,882,220 | 83,882,239 | ACTCGGCCTGCGCCGTGTCT |
| TDR | 83,882,159 | 83,882,179 | GCGGTAAAGTCCTGGGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGTAGGCTAGTGAAGAG |
| SD | TTTCCTGCCCAATTGATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
