10987 Spata9 Status: ES cell colonies screened / QC no positives
| Gene | Spata9 |
| Chromosome | 13 |
| Deletion Start | 75,967,818 |
| Deletion End | 75,998,771 |
| Deletion Size | 30,954 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,967,862 | 75,967,881 | GTGGACAGGTGGTGAAGAAC |
| TUP | 75,967,883 | 75,967,910 | TCTCTGGAAGACGTATGGCTGATCCTAC |
| TUR | 75,967,912 | 75,967,933 | CCATGCCCATAGTCCCAGTTAC |
| TDF | 75,998,060 | 75,998,082 | TCTGAAATCGGAAGGAGAGGAAG |
| TDP | 75,998,085 | 75,998,113 | AGCCCTGAGTGTTTACTGTCCTCACGTCA |
| TDR | 75,998,124 | 75,998,145 | CTAGTTTGCCTTGCAGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTTAGGGCAAAACTCAATC |
| SD | GGTAGGAGATATTTGAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
