10989   Lypd2     Status: ES cell colonies screened / QC no positives

GeneLypd2
Chromosome15
Deletion Start74,734,284
Deletion End74,732,334
Deletion Size1,951
Genome Build38
MGI IDMGI:1915561
NCBI Gene ID68311
BAC ID432B17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,733,88374,733,901GCCCGTTATGTGCTCAGTG
TUP74,733,85874,733,882ATGAGGGCTCAAGCCAGGCTGTTCC
TUR74,733,80574,733,823TGACCTGGTAGGGCCTCTG
TDF74,733,00174,733,022CCACTGCCACATCAATGAAACC
TDP74,732,97374,732,999TGTGCAAGACTACGCTCTACTCCCTGG
TDR74,732,94574,732,962GTCCCACGCAGATCCTAC

PCR Primers

PrimerSequence
SUGTGGGAAAAGAAAGACGACC
SDAAGCTTCTCCTCTAGCCCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev404 bp-++
TUFTUR97 bp++-
TDFTDR78 bp++-
NeoFwdSD360 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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