10990 Csgalnact2 Status: ES cell colonies screened / QC no positives
| Gene | Csgalnact2 |
| Chromosome | 6 |
| Deletion Start | 118,129,352 |
| Deletion End | 118,108,930 |
| Deletion Size | 20,423 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,129,133 | 118,129,151 | CAAACCAGGGCAACCAGTC |
| TUP | 118,129,112 | 118,129,132 | TGAAACGCCAGATTGCCCAGC |
| TUR | 118,129,067 | 118,129,087 | GCAAGGCTCTCATCTTCTCAC |
| TDF | 118,109,889 | 118,109,909 | CCCACAAGGGAAGGTCAGTAG |
| TDP | 118,109,865 | 118,109,888 | TCAGCAAGTCCAGCTGTGGTGAAC |
| TDR | 118,109,835 | 118,109,859 | TCGTGAATTTAGCTGTGTCTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGAAAAGTCACCACCCAG |
| SD | GCCTTTGTGATGGACTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 403 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 429 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
