10993   Dbpht2     Status: ES cell colonies screened / QC no positives

GeneDbpht2
Chromosome12
Deletion Start74,298,806
Deletion End74,299,201
Deletion Size396
Genome Build38
MGI IDMGI:2679513
NCBI Gene ID386753
BAC ID324H4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 12

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,298,84074,298,862GATGGAGACTCATGCAAGTATGC
TUP74,298,86874,298,887CAAACACACAGACATACAGA
TUR74,298,90574,298,927GTGTCTGTTTCTCTCATGTGTGC
TDF74,299,16174,299,181CCCCTGGTCCTTGATTTTCTC
TDPCACCTCCTTTCCTAGTG
TDR74,299,03774,299,055TGCACAGACACCCTCCCAA

PCR Primers

PrimerSequence
SUAGGAGTTGACAAGTGTTATC
SDACACAGCCTCAGGATTTAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev458 bp-++
TUFTUR88 bp++-
TDFTDR145 bp++-
NeoFwdSD396 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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