10994 Prrg3 Status: ES cell colonies screened / QC no positives
| Gene | Prrg3 |
| Chromosome | X |
| Deletion Start | 71,965,849 |
| Deletion End | 71,967,672 |
| Deletion Size | 1,824 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,966,521 | 71,966,540 | TGCCCATGCAGTCCTGAAAC |
| TUP | 71,966,544 | 71,966,569 | TTCCTCGTGCCAATGAGTTCCTGGAG |
| TUR | 71,966,584 | 71,966,604 | ATGCACTCCCGCTCAATGGTG |
| TDF | 71,967,156 | 71,967,179 | GTCTTGCTGATTGTCATTGCCTTG |
| TDP | 71,967,180 | 71,967,205 | TTTATCATCTGGAGGTGCCAACTGCA |
| TDR | 71,967,206 | 71,967,225 | GGTGGTGACGAGTTGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGGCTTGATTTCTCATCTG |
| SD | CTGCCTTGACCTCCTTATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
