10996 Arsg Status: Cancelled
| Gene | Arsg |
| Chromosome | 11 |
| Deletion Start | 109,490,416 |
| Deletion End | 109,517,422 |
| Deletion Size | 27,007 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,490,802 | 109,490,821 | ACAAGTGGGCGCTGGGAATC |
| TUP | 109,490,826 | 109,490,854 | CCAGGTCTCCTTTAAGTCAACAAGCATGC |
| TUR | 109,490,859 | 109,490,880 | GACGGGAGTGATGGCTCATTAG |
| TDF | 109,516,484 | 109,516,503 | TGGTCCCTTGTATGGCAGTG |
| TDP | 109,516,506 | 109,516,528 | TACCTCCTTGGAGCACCTGACGC |
| TDR | 109,516,535 | 109,516,559 | CCCTAAAGAAAGGATGGTCATGTTG |
PCR Primers
| Primer | Sequence |
| SU | CGATGAGAAGACATCTTGAG |
| SD | GGAAAACTCCCCAAGGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
