11004 Rab4b Status: Cancelled
| Gene | Rab4b |
| Chromosome | 7 |
| Deletion Start | 27,178,739 |
| Deletion End | 27,172,624 |
| Deletion Size | 6,116 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,178,154 | 27,178,176 | GGTTGGAAGGCAGTAGAAATCTC |
| TUP | 27,178,126 | 27,178,153 | TGACGACAATCAGAGACAGCTCCATTCA |
| TUR | 27,178,040 | 27,178,059 | CCTCTGACGTTGGGCCTATG |
| TDF | 27,173,377 | 27,173,396 | TTTGCTGCTAGGCAGATGTG |
| TDP | 27,173,345 | 27,173,372 | CGAATCCTGAGTACAACTTGAGCCTCCA |
| TDR | 27,173,310 | 27,173,328 | CAAGCCCACCAGAGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GCTCCTTCTTGGCATTCTTG |
| SD | ATTGCAGCAGTGATAACCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
