11005   Bola3     Status: ES cell colonies screened / QC positives

GeneBola3
Chromosome6
Deletion Start83,349,487
Deletion End83,358,213
Deletion Size8,727
Genome Build38
MGI IDMGI:1925903
NCBI Gene ID78653
BAC ID258H7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF83,350,40083,350,417CCGCATCTCAGCCATCTC
TUP83,350,42083,350,445AGGCATCTTGGCCTAATGTCCAGTGA
TUR83,350,45683,350,473GCAGCGAATGAGCAGGTG
TDF83,357,32783,357,346TGCTGGACTGGACTCCTGAG
TDP83,357,34783,357,368CTCAAGCTGCCACCTGGCCTCA
TDR83,357,42783,357,448CACCCAGCTCCCTTTACGATTG

PCR Primers

PrimerSequence
SUCAACCTGTTCTGTGCACCC
SDCAGGAGTAGGATGAACTGTAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev374 bp-++
TUFTUR74 bp++-
TDFTDR122 bp++-
NeoFwdSD353 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++