11005 Bola3 Status: ES cell colonies screened / QC positives
| Gene | Bola3 |
| Chromosome | 6 |
| Deletion Start | 83,349,487 |
| Deletion End | 83,358,213 |
| Deletion Size | 8,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,350,400 | 83,350,417 | CCGCATCTCAGCCATCTC |
| TUP | 83,350,420 | 83,350,445 | AGGCATCTTGGCCTAATGTCCAGTGA |
| TUR | 83,350,456 | 83,350,473 | GCAGCGAATGAGCAGGTG |
| TDF | 83,357,327 | 83,357,346 | TGCTGGACTGGACTCCTGAG |
| TDP | 83,357,347 | 83,357,368 | CTCAAGCTGCCACCTGGCCTCA |
| TDR | 83,357,427 | 83,357,448 | CACCCAGCTCCCTTTACGATTG |
PCR Primers
| Primer | Sequence |
| SU | CAACCTGTTCTGTGCACCC |
| SD | CAGGAGTAGGATGAACTGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
