11005 Bola3 Status: ES cell colonies screened / QC positives
Gene | Bola3 |
Chromosome | 6 |
Deletion Start | 83,349,487 |
Deletion End | 83,358,213 |
Deletion Size | 8,727 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 83,350,400 | 83,350,417 | CCGCATCTCAGCCATCTC |
TUP | 83,350,420 | 83,350,445 | AGGCATCTTGGCCTAATGTCCAGTGA |
TUR | 83,350,456 | 83,350,473 | GCAGCGAATGAGCAGGTG |
TDF | 83,357,327 | 83,357,346 | TGCTGGACTGGACTCCTGAG |
TDP | 83,357,347 | 83,357,368 | CTCAAGCTGCCACCTGGCCTCA |
TDR | 83,357,427 | 83,357,448 | CACCCAGCTCCCTTTACGATTG |
PCR Primers
Primer | Sequence |
SU | CAACCTGTTCTGTGCACCC |
SD | CAGGAGTAGGATGAACTGTAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 374 bp | - | + | + |
TUF | TUR | 74 bp | + | + | - |
TDF | TDR | 122 bp | + | + | - |
NeoFwd | SD | 353 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |