11010 Slc2a13 Status: Cancelled
| Gene | Slc2a13 |
| Chromosome | 15 |
| Deletion Start | 91,573,037 |
| Deletion End | 91,572,565 |
| Deletion Size | 473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,572,912 | 91,572,928 | CGCGCAGGCAGTTCCAG |
| TUP | 91,572,886 | 91,572,907 | ACGAGACCCCGGCCTTCGTGTA |
| TUR | 91,572,827 | 91,572,842 | CCACGCCGGTGTCGTA |
| TDF | 91,572,675 | 91,572,690 | GCGCAGCGCCATCCTG |
| TDP | 91,572,651 | 91,572,669 | CAGCGCCCTGTGCACCGTG |
| TDR | 91,572,606 | 91,572,625 | CAGCAGCGTCTCCTTGTTGG |
PCR Primers
| Primer | Sequence |
| SU | GAGTTCTTCTCTGTCTCCGC |
| SD | GATGGCAGGGATGTGGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 354 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
