11015 Cldn18 Status: Germline transmission achieved
| Gene | Cldn18 |
| Chromosome | 9 |
| Deletion Start | 99,610,364 |
| Deletion End | 99,593,064 |
| Deletion Size | 17,301 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,610,067 | 99,610,085 | GCAATCCGCCTCAGGTGTG |
| TUP | 99,610,043 | 99,610,066 | ACTGTGGCAGCCTTGGCTCCCAAT |
| TUR | 99,609,977 | 99,610,001 | TCTCCTATCTCCTACATGGCTACTG |
| TDF | 99,593,330 | 99,593,349 | GCACAGGTGTTCATGGACTC |
| TDP | 99,593,302 | 99,593,328 | TTCCTTGCCTTTCAGCTTCAAAGCTGT
|
| TDR | 99,593,282 | 99,593,301 | GGCCAGAGGCATGGTAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGGGAGAGTAGGTTCGTCC |
| SD | AAAATCGACCATCAGCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 49 bp | + | + | - |
| NeoFwd | SD | 402 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11015B-H4 | VGB6 | C57BL/6NTac | |
| 11015B-G8 | VGB6 | C57BL/6NTac | |
| 11015C-H11 | VGB6 | C57BL/6NTac | |
| 11015C-H3 | VGB6 | C57BL/6NTac | |
| 11015D-E7 | VGB6 | C57BL/6NTac | |
| 11015D-F3 | VGB6 | C57BL/6NTac | |
