11019 Ier5l Status: ES cell colonies screened / QC no positives
| Gene | Ier5l |
| Chromosome | 2 |
| Deletion Start | 30,474,008 |
| Deletion End | 30,472,748 |
| Deletion Size | 1,261 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,473,898 | 30,473,920 | ACAAGAACCTCCTGGTGTCCTAC |
| TUP | | | TCCGCAACGCGCGCCAG |
| TUR | 30,473,842 | 30,473,860 | CGGTAGAGCTCGGCGTAAC |
| TDF | 30,472,969 | 30,472,987 | AGGACTTCTGCCCGGACTC |
| TDP | 30,472,931 | 30,472,959 | CGCGTCCAACATCTCAAACTTGATCTCCA |
| TDR | 30,472,911 | 30,472,929 | CGAGAAGCCCGAGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAATAGCACAAAGGAACCGC |
| SD | TTTCACAAGACCCGTTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
