11023 Zfp488 Status: ES cell colonies screened / QC no positives
| Gene | Zfp488 |
| Chromosome | 14 |
| Deletion Start | 33,971,150 |
| Deletion End | 33,970,172 |
| Deletion Size | 979 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,971,037 | 33,971,056 | TGAGCCTTGTGGACTCTGAC |
| TUP | 33,971,010 | 33,971,035 | CTGCTGCAGGAAAGGGTAGCCAAGAT |
| TUR | 33,970,967 | 33,970,985 | GCTTGTTCGGTGCTGTTGG |
| TDF | 33,970,399 | 33,970,419 | ACTCTCCTCCTTGGGCTTGTC |
| TDP | 33,970,372 | 33,970,397 | ACTCAGAACTGGTGTGCGAAGTGCAA |
| TDR | 33,970,324 | 33,970,343 | TGTGACCGCATGTGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTTTTGGATGACAATGCTCC |
| SD | ACTGGGGACAGAGACAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 413 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 366 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
