11027 Mageb18 Status: Cancelled
| Gene | Mageb18 |
| Chromosome | X |
| Deletion Start | 92,120,631 |
| Deletion End | 92,119,651 |
| Deletion Size | 981 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,120,526 | 92,120,547 | CCAGGTCCTTTCTATGGGTGTC |
| TUP | 92,120,499 | 92,120,525 | CACATCAGGCTAGTCCTGTTGCCAGTA |
| TUR | 92,120,473 | 92,120,495 | CATTGGCATGTTGGGAGATGTAG |
| TDF | 92,119,715 | 92,119,733 | CTGTAGCCAGGGCTCACAC |
| TDP | 92,119,690 | 92,119,714 | TGCTGCAATGGCCAGTCCATATTCA |
| TDR | 92,119,661 | 92,119,680 | GTATGGGAGGAGCTGCACAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGTCAAGGTATCTTCTGG |
| SD | GATTTGAAACTTAGGTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 411 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
