11028 Cpxcr1 Status: ES cell colonies screened / QC no positives
| Gene | Cpxcr1 |
| Chromosome | X |
| Deletion Start | 116,477,397 |
| Deletion End | 116,478,407 |
| Deletion Size | 1,011 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,477,477 | 116,477,499 | TGAGAAAGAACACCCACTTCCTG |
| TUP | 116,477,511 | 116,477,538 | TCTCAGGTGGAAACTAATCTATCCGGCA |
| TUR | 116,477,545 | 116,477,569 | TCCTCCTGAGAACTTATTGAGCTTG |
| TDF | 116,478,286 | 116,478,306 | TGTGTCCCATTTGTGGTTGTG |
| TDP | 116,478,308 | 116,478,329 | TTTCAACAACTTTTATGATTTT |
| TDR | 116,478,333 | 116,478,356 | GCCAGAAAAGCTGCAAGAATGATG |
PCR Primers
| Primer | Sequence |
| SU | AAATTTGACATCTAAAATGG |
| SD | CTATTGTACCTTTCTCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 501 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 399 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
