11029 Spesp1 Status: Cancelled
| Gene | Spesp1 |
| Chromosome | 9 |
| Deletion Start | 62,282,059 |
| Deletion End | 62,272,341 |
| Deletion Size | 9,719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,282,027 | 62,282,045 | AGTTGCGCTGTGGCTATGG |
| TUP | 62,282,003 | 62,282,026 | CCTTCGTCACTGCTGGCGTATCCA |
| TUR | 62,281,980 | 62,281,997 | CACCACCAGGCACATCAC |
| TDF | 62,273,079 | 62,273,101 | CAGAGCCCGAAGAAGAGTTAGTG |
| TDP | 62,273,053 | 62,273,077 | CCAGCACAACGCCAAACAAGGAGCT |
| TDR | 62,272,992 | 62,273,011 | ACGAGTTGCCTGGGTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | ACATCCACTTCTGTGCAACG |
| SD | ATGGCCTTCATCACAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 457 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 476 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
