| Gene | Ccdc115 |
| Chromosome | 1 |
| Deletion Start | 34,439,335 |
| Deletion End | 34,437,496 |
| Deletion Size | 1,840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,438,830 | 34,438,853 | GCATGTAAGTCTCCTTTCCACCTG |
| TUP | 34,438,804 | 34,438,829 | TCAGCCCTTACTCAGCGCTAAAGACA |
| TUR | 34,438,735 | 34,438,753 | CTCCAGCTCAGACGCTCTC |
| TDF | 34,437,754 | 34,437,773 | TTTGGTTCCTCACAGTCTGC |
| TDP | 34,437,726 | 34,437,747 | CCCAAGCCAGCTTCCGGGATGG |
| TDR | 34,437,692 | 34,437,713 | GCCTCCCTAGCCAGTACAATAG |
PCR Primers
| Primer | Sequence |
| SU | GGTAGCCATCGGATTCGAG |
| SD | TTCACTATCCCAGATGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11032B-C1 | VGB6 | C57BL/6NTac | |
| 11032B-F9 | VGB6 | C57BL/6NTac | |
