11039 Ubqln2 Status: Cancelled
| Gene | Ubqln2 |
| Chromosome | X |
| Deletion Start | 153,498,440 |
| Deletion End | 153,500,647 |
| Deletion Size | 2,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,498,880 | 153,498,898 | CCCAGGAGTAGCTCCACAC |
| TUP | 153,498,901 | 153,498,926 | ACCACCACGAATAGCAGCTCCTTTGG |
| TUR | 153,498,950 | 153,498,969 | CGAGTTCAAGCCCAGGTTAC |
| TDF | 153,499,827 | 153,499,846 | GGCCATGTCAAACCCGAGAG |
| TDP | 153,499,847 | 153,499,871 | CGATGCAAGCGCTAATGCAGATCCA |
| TDR | 153,499,884 | 153,499,905 | AGGTGCTTCAGTGGCTAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGACGGCCTGCAGGACCTG |
| SD | CATGCCAAAGTCACAATAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
