11044 Defb34 Status: Cancelled
| Gene | Defb34 |
| Chromosome | 8 |
| Deletion Start | 19,123,755 |
| Deletion End | 19,126,541 |
| Deletion Size | 2,787 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,123,822 | 19,123,842 | TATGGAGGGTGACAGGAGGTC |
| TUP | 19,123,845 | 19,123,873 | TCCTGAAAATGTTCACACGTGATCAACCC |
| TUR | 19,123,884 | 19,123,907 | CAAGTCCAACACACCAAAGGTATC |
| TDF | 19,125,824 | 19,125,846 | TGGCCCATTGGAAACACATAGAC |
| TDP | 19,125,855 | 19,125,884 | TCTTGGTCTTCAATCTTCCTGGGTCATCAC |
| TDR | 19,125,908 | 19,125,930 | TGGAGAAGACAGCAAGTGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGTGAGAATTAGCAATGC |
| SD | TGCTCATTTCTCCACATATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 467 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 382 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
