11050 Spata3 Status: Cancelled
| Gene | Spata3 |
| Chromosome | 1 |
| Deletion Start | 86,022,033 |
| Deletion End | 86,026,557 |
| Deletion Size | 4,525 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,022,243 | 86,022,264 | ACCGCAACAGCATACACCACAG |
| TUP | 86,022,265 | 86,022,288 | CCTCTCCCGCCTCCAGAGAAACCT |
| TUR | 86,022,320 | 86,022,343 | TCCTGCTTGGAAGAATTGGTTTAG |
| TDF | 86,026,162 | 86,026,181 | TGGGAGGTGTCTGGAACAAG |
| TDP | 86,026,182 | 86,026,205 | ACCTGGCACATCGTGGTCTGGACC |
| TDR | 86,026,233 | 86,026,251 | CGGTAGCCTGCGACTGATG |
PCR Primers
| Primer | Sequence |
| SU | ATGGATCCAGTTCTCTTAGG |
| SD | TGGTACAGGCGGGAGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 395 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
