11051 Ggn Status: ES cell colonies screened / QC no positives
| Gene | Ggn |
| Chromosome | 7 |
| Deletion Start | 29,172,808 |
| Deletion End | 29,173,943 |
| Deletion Size | 1,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,172,955 | 29,172,973 | AGCAACGGAACCTCCACAG |
| TUP | 29,172,980 | 29,173,005 | CCTCTAACAAGGGAACAGCAAGGCAC |
| TUR | 29,173,013 | 29,173,036 | GACAGGAATTAAGCACCTCGAAAG |
| TDF | 29,173,427 | 29,173,446 | GGGACCTGAGGGTGGATTAG |
| TDP | 29,173,448 | 29,173,472 | AAGCCTCCAATTTGCCTGGTCCCAT |
| TDR | 29,173,474 | 29,173,492 | TACGGGCACTGGGAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCCAGGTCAGGTCAGACAC |
| SD | GCTACCATTTGAGCCCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
