| Gene | Dusp28 |
| Chromosome | 1 |
| Deletion Start | 92,907,039 |
| Deletion End | 92,907,687 |
| Deletion Size | 649 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,907,260 | 92,907,279 | ACCTGCTGACACACCTGGAG |
| TUP | 92,907,280 | 92,907,301 | CCCACCTGTGCCGCCATGGAAG |
| TUR | 92,907,331 | 92,907,349 | GGCCGTTCTTGCAGTACAC |
| TDF | 92,907,510 | 92,907,529 | CCTCTGCACCCTGTACTGTC |
| TDP | 92,907,532 | 92,907,554 | TCGAGTCTCTCACCGCAGCTCTC |
| TDR | 92,907,622 | 92,907,643 | GAAGGGTCTGCTCGTACTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGAATAGAGCTGCAACTGAC |
| SD | AGGCAGCTAGGACTCTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11053A-F2 | VGB6 | C57BL/6NTac | |
| 11053A-D3 | VGB6 | C57BL/6NTac | |
| 11053A-D12 | VGB6 | C57BL/6NTac | |
| 11053A-B12 | VGB6 | C57BL/6NTac | |
