11059   Lypd1     Status: ES cell colonies screened / QC no positives

GeneLypd1
Chromosome1
Deletion Start125,911,887
Deletion End125,910,376
Deletion Size1,512
Genome Build38
MGI IDMGI:1919835
NCBI Gene ID72585
BAC ID83D15
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,911,711125,911,729GCTACCCAGCGGGAGTTTG
TUP125,911,683125,911,706TGGAAACTGAGACCAAAGGCGCTA
TUR125,911,665125,911,682TTGGAGAGCGTCCCAGTC
TDF125,910,627125,910,647AGAGCCCAGAAGCCCTTGTTG
TDP125,910,596125,910,619CAGTACACGGTGATACCGGCCAGC
TDR125,910,574125,910,592GGCTTGCACAGAGCACATC

PCR Primers

PrimerSequence
SUGATCGCCAGTAGTTCTTCAAG
SDGGAGTCCCCTGGGAAGAAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev409 bp-++
TUFTUR65 bp++-
TDFTDR74 bp++-
NeoFwdSD362 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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