11060 Spaca4 Status: ES cell colonies screened / QC no positives
| Gene | Spaca4 |
| Chromosome | 7 |
| Deletion Start | 45,725,573 |
| Deletion End | 45,725,137 |
| Deletion Size | 437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,725,528 | 45,725,547 | GTTGGTTCTTTGCCCAGGTG |
| TUP | 45,725,505 | 45,725,527 | TGACAGGCATCAAGGACTGCGTC |
| TUR | 45,725,482 | 45,725,502 | GCAGAGTCAGTCAGCTCACAG |
| TDF | 45,725,302 | 45,725,319 | CACCACCACCTGCTGTTC |
| TDP | 45,725,279 | 45,725,301 | TGGCCACCTTTGCAATAAGGGCA |
| TDR | 45,725,189 | 45,725,210 | TGGGTCACAGCCAGTATTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCCCAAAGGCTTGTGAGCAG |
| SD | ACACCAACATAAAGCAGCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 131 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
