11062 Pdha2 Status: ES cell colonies screened / QC no positives
| Gene | Pdha2 |
| Chromosome | 3 |
| Deletion Start | 141,211,742 |
| Deletion End | 141,210,521 |
| Deletion Size | 1,222 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,211,307 | 141,211,325 | GGACGCAAAGGAGGCTGTG |
| TUP | 141,211,281 | 141,211,305 | TAAAGGCAAGGGAGGCTCCATGCAC |
| TUR | 141,211,248 | 141,211,266 | GCCATTGCCACCGTAGAAG |
| TDF | 141,210,961 | 141,210,985 | GGGTGAATGGGATGGATATTCTCTG |
| TDP | 141,210,936 | 141,210,958 | TTCGGGAGGCAACCAAGTTTGCA |
| TDR | 141,210,847 | 141,210,867 | ATCCCTGGGTCGCTCATACTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGTGAGTGGCAACCATG |
| SD | AACCAAATGAAACTACTATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | 381 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
