11063 Pnma2 Status: Cancelled
| Gene | Pnma2 |
| Chromosome | 14 |
| Deletion Start | 66,916,132 |
| Deletion End | 66,917,315 |
| Deletion Size | 1,204 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,916,472 | 66,916,491 | AGACGGTCGCAGGGATGTTC |
| TUP | 66,916,492 | 66,916,518 | CGAGCCCTTAAGCATGAGGGAGTGTCT |
| TUR | 66,916,546 | 66,916,567 | CCGTCAAATGGGCCAATAACTC |
| TDF | 66,917,018 | 66,917,037 | AGGTCATGGCTGGTGCCAAC |
| TDP | 66,917,038 | 66,917,059 | CTCGGCAACGTTCTGTGGTGCA |
| TDR | 66,917,065 | 66,917,085 | GCCCTGATCTTTCAGCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGAAGTAGAGGGCTGTG |
| SD | TGCTCCCTCTGAAGAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 553 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 412 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
