11068 Ccdc54 Status: Cancelled
| Gene | Ccdc54 |
| Chromosome | 16 |
| Deletion Start | 50,590,902 |
| Deletion End | 50,589,915 |
| Deletion Size | 988 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,590,785 | 50,590,805 | CAGAAATGTACGACCCACCAC |
| TUP | 50,590,753 | 50,590,778 | TCAACTAGCTACCCAACAAGGGCTTC |
| TUR | 50,590,728 | 50,590,748 | GAGAGTATCTCCGTCGCAACC |
| TDF | 50,590,375 | 50,590,398 | GCTCCTCCATAAACTCCTACAAAC |
| TDP | 50,590,348 | 50,590,368 | CCCAAAGGGCACAGCCACCTC |
| TDR | 50,590,318 | 50,590,339 | GGCTCTGCTGATGAGATGACAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGACATCACAATCAGATG |
| SD | GAACGGATGTGGAAGCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 385 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
