11070 Ppp1r3b Status: ES cell colonies screened / QC no positives
| Gene | Ppp1r3b |
| Chromosome | 8 |
| Deletion Start | 35,384,012 |
| Deletion End | 35,384,885 |
| Deletion Size | 874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,384,165 | 35,384,188 | GTGCAGGAGAAGAAGGTGAAGAAG |
| TUP | 35,384,189 | 35,384,210 | CGGGTGTCCTTCGCCGACAACC |
| TUR | 35,384,294 | 35,384,314 | CGTCAGACTCACGATGTTGTC |
| TDF | 35,384,558 | 35,384,577 | GCTGGTTCAGACAGGGACAC |
| TDP | 35,384,580 | 35,384,606 | TCTCCTTTGACATCAGCTTACCCGAGA |
| TDR | 35,384,630 | 35,384,648 | CGTAGCACACGGCAAACTC |
PCR Primers
| Primer | Sequence |
| SU | TGGTGTATTAGCATCCTGGC |
| SD | CTCGAGGTCAATGGTAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
