11079 Igbp1b Status: ES cell colonies screened / QC no positives
| Gene | Igbp1b |
| Chromosome | 6 |
| Deletion Start | 138,658,441 |
| Deletion End | 138,657,369 |
| Deletion Size | 1,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,658,289 | 138,658,309 | GCCTTGAAGCTCCTGGAGAAG |
| TUP | 138,658,263 | 138,658,287 | CCTCGGACATGTTATCGCAGCTCGA |
| TUR | 138,658,213 | 138,658,232 | TTAGGTCAGCGGAAGCAATC |
| TDF | 138,657,547 | 138,657,570 | CACCACCATCTGAGACTTTCACTG |
| TDP | 138,657,519 | 138,657,544 | TCTGAGAAGGAAGAGCCAGACCTGGA |
| TDR | 138,657,466 | 138,657,485 | CATCCCACTCCTGCATCCTG |
PCR Primers
| Primer | Sequence |
| SU | GCCTAGCAATGGTTACCATG |
| SD | TGAAACTTACGTGAATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 417 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
